Co-regulation of multiple genes promote inner ear progenitors to regenerate hair cells in the mouse cochlea
编号：123
访问权限：仅限参会人
更新：2022-07-06 17:10:14
浏览：383次
主旨报告
报告开始：2022年07月24日 11:15 （Asia/Shanghai）
报告时间：25min
所在会议：[P] 全体会议 » [P-2] 主旨报告2
暂无文件
摘要
Sensory hair cells in the inner ear are responsible for sound recognition. Damage to hair cells in adult mammals causes permanent hearing impairment because these cells cannot regenerate. By contrast, newborn mammals possess limited regenerative capacity because of the active participation of various signaling pathways. The Wnt, Notch and Atoh1 are highly sophisticated and conserved signaling pathways that control multiple cellular events necessary for the formation of sensory hair cells. All three signlaings allow resident supporting cells to regenerate hair cells in the neonatal cochlea. Foxg1 is one of the forkhead genes that are involved in morphogenesis, cell fate determination, and proliferation, and Foxg1 was previously reported to be required for morphogenesis of the mammalian inner ear. We found that Foxg1 conditional knock-down (cKD) in Sox2+ SCs and Lgr5+ progenitors at postnatal day (P)1 both led to large numbers of extra inner HCs in P7 mouse cochleae, and these extra inner HCs survived at least to P30 and had normal hair bundles and synapses. Pou4f3 and Gfi1, are orderly expressed in the process of HC differentiation and maturation. The Pou4f3 gene is an essential gene for HC formation in the cochlear development, and also is a target of ATOH1 in HCs. Gfi1 is a target of the Pou4f3 gene, and is essential for the survival of cochlear HC. Considering the possible roles of these geens in mammalian hair cell regeneration and maturation, we co-regulated these genes simultaneously in postnatal cochlear supporting cells, and explored the roles of these genes in SC-to-HC conversion and maturation of new HCs.
关键字
报告人
柴人杰
东南大学柴人杰，东南大学首席教授、二级教授，东南大学生命健康高等研究院执行院长、生命科学与技术学院副院长、附属中大医院耳鼻喉科双聘教授；教育部长江学者特聘教授，国家重点研发计划首席科学家，青年千人，国家优青。现任中国生物物理学会听觉分会会长；中国生理学会干细胞生理专委会副主委、候任主委；中国听基会基础研究专委会主委, ESCI期刊American Journal of Stem Cells执行主编。长期致力于神经元和内耳毛细胞的再生和保护研究近5年发表SCI论文118篇（总影响因子1177.13）；其中通讯作者论文105篇（总影响因子955.31，其中IF>30分4篇，IF 10-30分23篇, IF 8-10分9篇），其中4篇论文被Faculty1000列为推荐文章，1篇论文入选Cell正刊年度最佳论文，10篇论文入选了ESI高被引论文，2篇论文入选热点论文，总他引4800余次，h-index 41；先后获教育部霍英东青年科学奖一等奖，教育部自然科学奖一等奖，江苏省医学科技奖一等奖，上海市技术发明奖一等奖，树兰医学青年奖，江苏省青年科技奖和江苏省青年十大科技之星等奖励。
发表评论